Friday, July 31, 2015

Questions


A  Is there an identifiable odor a) glycemic event  b) seizure  
  1. During a low glycemic event does the body produce an odor that can be detected in the/on the  saliva by dogs trained to identify chemical changes in the body
  2. During a low glycemic event does the body produce an odor that can be detected in the/on the  skin swab by dogs trained to identify chemical changes in the body
  3. During a  tonic clonic seizure event does the body produce an odor that can be detected in the/on the  saliva by dogs trained to identify chemical changes in the body
  4. During a  tonic clonic seizure event does the body produce an odor that can be detected in the/on the  skin swab by dogs trained to identify chemical changes in the body


B  Can odor be captured a) from different sources b) on different medium
  1. Can odor produced during a low glycemic event  be captured from saliva placed on  dental cotton and be detected by dogs trained to identify specific odors
  2. Can odor produced during a  low glycemic event  be captured from skin swab placed on  gauze pads be detected by dogs trained to identify specific odors
  3. Can odor produced during a   tonic clonic seizure  be captured from saliva placed on  dental cotton be detected by dogs trained to identify specific odors
  4. Can odor produced during a   tonic clonic seizure  be captured from skin swab placed on  gauze pads be detected by dogs trained to identify specific odors
  5. Can odor produced during a   tonic clonic seizure  be captured from seizure event placed on  cotton pads be detected by dogs trained to identify specific odors


C  Is there a difference between event odor and non-event odor
  1. Can a dog trained to identify chemical changes in the body distinguish between the odor of the saliva  in a person experiencing a low glycemic event vs the odor of the saliva of the same person in a euglycemic state
  2.  Can a dog trained to identify chemical changes in the body distinguish between the odor of the skin swab  in a person experiencing a low glycemic event vs the odor of the skin swab of the same person in a euglycemic state
  3. Can a dog trained to identify chemical changes in the body distinguish between the odor of the saliva  in a person experiencing a  tonic clonic seizure event vs the odor of the saliva of the same person in a non-seizure state
  4. Can a dog trained to identify chemical changes in the body distinguish between the odor of the skin swab  in a person experiencing a  tonic clonic seizure event  vs the odor of the skin swab of the same person in a non-seizure-state          

D  Can viable sample be replicated through hydration
  1. Can odor  produced during a low glycemic event  be captured from saliva placed on dental cotton  be replicated via hydration be identified by dogs trained to identify chemical changes in the body
  2. Can odor  produced during a low glycemic event  be captured from skin swab placed on gauze pads  be replicated via hydration be identified by dogs trained to identify chemical changes in the body
  3. Can odor  produced during a  tonic clonic seizure event  be captured from saliva placed on dental cotton  be replicated via hydration be identified by dogs trained to identify chemical changes in the body
  4. Can odor  produced during a  tonic clonic seizure event event  be captured from skin swab placed on gauze pads  be replicated via hydration be identified by dogs trained to identify chemical changes in the body


E  Can viable sample replicated through hydration be identified after being frozen
  1. Can odor  produced during a low glycemic event  be captured from saliva placed on dental cotton  be replicated via hydration then frozen be identified by dogs trained to identify chemical changes in the body
  2. Can odor  produced during a low glycemic event  be captured from skin swab placed on gauze pads  be replicated via hydration then frozen be identified by dogs trained to identify chemical changes in the body
  3. Can odor  produced during a  tonic clonic seizure event  be captured from saliva placed on dental cotton  be replicated via hydration then frozen be identified by dogs trained to identify chemical changes in the body
  4. Can odor  produced during a  tonic clonic seizure event event  be captured from skin swab placed on gauze pads  be replicated via hydration then frozen be identified by dogs trained to identify chemical changes in the body


Future Tests:
  • all of the above testing viability after a certain length of time
  • all of the above testing viability after storage variables (refrigeration, freezing, etc) 
  • common identifiable biological marker between LowBG and pre-ictal seizure event
  • common identifiable marker between types of seizures
  • common identifiable marker between pre and post ictal seizure sample
  • can dog discriminate between sample donors
  • between species
  • odor and stress events (panic attacks, PTSD, etc) 

Non-wheel tests:
(using Nosework hide techniques)
  • can odor be detected in the air; on clothing
  • from a distance; a measurable distance

Question A I



Question
During a  hypoglycemic event does the body produce an odor in the/on the  saliva that can be detected by dogs trained to identify chemical changes in the body

Research

Hypothesis


Methods/Procedures
Equipment
  • 24" scent wheel 
  • 6 containers (clean tin cans)
  • 6 glass shakers w.metal lids
    • variable numbers of holes in lids
  • Lazy Susan
Protocol
  • run containers (cans) and shakers twice through the dishwasher prior to test
  • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
  • store in Ziplock plastic bags when not in use
    • store "hot" shaker separately in its own Ziplock bag
Testing Instrument
  • dog
  • dog training
    • Scent Discrimination
    • Intro to Scent Wheel
    • Qualifying Test Protocol
    • Amended Testing Progression
    • Trial Test

Data
    • Dog 5 Donor 5  DoC  9/24/13
      • BCM
    • Dog 6 Donor 7  DoC  11/20/13
      • BCM
    • Dog 3 Donor 7  DoC 11/20/13
      • 60%  
    • Dog 5 Donor 7  DoC 11/20/13
      • 100%

    Observations


    Conclusion

    low BG IDd by saliva sample 

    Question A II



    Question
    During a  hypoglycemic event does the body produce an odor in the/on the skin (neck/forehead) that can be detected by dogs trained to identify chemical changes in the body

    Research

    Hypothesis
    During a glycemic event the body produces an odor on the skin (neck/forehead) that can be detected by a dog trained to identify chemical changes in the body 


    Methods/Procedures
    Equipment
    • 24" scent wheel 
    • 6 containers (clean tin cans)
    • 6 glass shakers w.metal lids
      • variable numbers of holes in lids
    • Lazy Susan
    Protocol
    • run containers (cans) and shakers twice through the dishwasher prior to test
    • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
    • store in Ziplock plastic bags when not in use
      • store "hot" shaker separately in its own Ziplock bag
    Testing Instrument
    • dog
    • dog training
      • Scent Discrimination
      • Intro to Scent Wheel
      • Qualifying Test Protocol
      • Amended Testing Progression
      • Trial Test

    Data
    • Dog 5 Donor 7  DoC 11/20/13
      • BCM
    • Dog 5 Donor 5  Doc  2/5/14
      • BCM


    Observations


    Conclusion

    low BG  IDd by skin swab

    Question A III



    Question
    During a  hypoglycemic event does the body produce an odor in the/on the skin (neck/forehead) that can be detected by dogs trained to identify chemical changes in the body

    Research

    Hypothesis
    During a glycemic event the body produces an odor on the skin (neck/forehead) that can be detected by a dog trained to identify chemical changes in the body 


    Methods/Procedures
    Equipment
    • 24" scent wheel 
    • 6 containers (clean tin cans)
    • 6 glass shakers w.metal lids
      • variable numbers of holes in lids
    • Lazy Susan
    Protocol
    • run containers (cans) and shakers twice through the dishwasher prior to test
    • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
    • store in Ziplock plastic bags when not in use
      • store "hot" shaker separately in its own Ziplock bag
    Testing Instrument
    • dog
    • dog training
      • Scent Discrimination
      • Intro to Scent Wheel
      • Qualifying Test Protocol
      • Amended Testing Progression
      • Trial Test

    Data
    • Dog 5 Donor 7  DoC 11/20/13
      • BCM
    • Dog 5 Donor 5  Doc  2/5/14
      • BCM


    Observations


    Conclusion

    low BG  IDd by skin swab

    Question A IV



    Question
    During a  tonic clonic seizure event does the body produce an odor that can be detected in the/on the  skin swab by dogs trained to identify chemical changes in the body

    Research

    Hypothesis
    The body produces a unique identifiable odor as a result of chemical changes in the body during a tonic clonic seizure

    Methods/Procedures
    Equipment
    • 24" scent wheel 
    • 6 containers (clean tin cans)
    • 6 glass shakers w.metal lids
      • variable numbers of holes in lids
    • Lazy Susan
    Protocol
    • run containers (cans) and shakers twice through the dishwasher prior to test
    • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
    • store in Ziplock plastic bags when not in use
      • store "hot" shaker separately in its own Ziplock bag
    Testing Instrument
    • dog
    • dog training
      • Scent Discrimination
      • Intro to Scent Wheel
      • Qualifying Test Protocol
    Testing Procedures
    • Amended Testing Progression

    Data
    • Dog 3 Donor 3  DoC 8/19/12
      • BCM

    Observations
    • Need 2 more tests 

    Conclusion

    seizure IDd by skin swab

    Question B I



    Question
    Can odor produced during a   hypoglycemic event  be captured from saliva placed on  dental cotton and be detected by dogs trained to identify specific odors





          Research


          • ICAN Study
          • A I

          Hypothesis


          Methods/Procedures


          Equipment
          • 24" scent wheel 
          • 6 containers (clean tin cans)
          • 6 glass shakers w.metal lids
            • variable numbers of holes in lids
          • Lazy Susan
          Protocol
          • run containers (cans) and shakers twice through the dishwasher prior to test
          • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
          • store in Ziplock plastic bags when not in use
            • store "hot" shaker separately in its own Ziplock bag
          Testing Instrument
          • dog
          • dog training
            • Scent Discrimination
            • Intro to Scent Wheel
            • Qualifying Test Protocol
            • Amended Testing Progression
            • Trial Test

          Data
          • Dog 5 Donor 5  DoC  9/24/13
            • BCM
          • Dog 6 Donor 7  DoC  11/20/13
            • BCM


          Observations


          Conclusion

          Cotton pads, dental cotton

          Question B II



          Question
          Can odor produced during a   hypoglycemic event  be captured from skin swab placed on gauze pads be detected by dogs trained to identify specific odors


              Research


              • ICAN Study
              • A II

              Hypothesis


              Methods/Procedures


              Equipment
              • 24" scent wheel 
              • 6 containers (clean tin cans)
              • 6 glass shakers w.metal lids
                • variable numbers of holes in lids
              • Lazy Susan
              Protocol
              • run containers (cans) and shakers twice through the dishwasher prior to test
              • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
              • store in Ziplock plastic bags when not in use
                • store "hot" shaker separately in its own Ziplock bag
              Testing Instrument
              • dog
              • dog training
                • Scent Discrimination
                • Intro to Scent Wheel
                • Qualifying Test Protocol
                • Amended Testing Progression
                • Trial Test

              Data
              • Dog 5 Donor 7  DoC 11/20/13
                • BCM
              • Dog 5 Donor 5  Doc  2/5/14
                • BCM


              Observations


              Conclusion

              gauze pads

              Question B III



              Question


              Can odor produced during a   tonic clonic seizure  be captured from saliva placed on  dental cotton be detected by dogs trained to identify specific odors
                Can odor produced during a   tonic clonic seizure  be captured from seizure event placed on  cotton pads be detected by dogs trained to identify specific odors

                  Research


                  • Research Index
                  • A III


                  Hypothesis


                  Methods/Procedures


                  Equipment
                  • 24" scent wheel 
                  • 6 containers (clean tin cans)
                  • 6 glass shakers w.metal lids
                    • variable numbers of holes in lids
                  • Lazy Susan
                  Protocol
                  • run containers (cans) and shakers twice through the dishwasher prior to test
                  • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
                  • store in Ziplock plastic bags when not in use
                    • store "hot" shaker separately in its own Ziplock bag
                  Testing Instrument
                  • dog
                  • dog training
                    • Scent Discrimination
                    • Intro to Scent Wheel
                    • Qualifying Test Protocol
                    • Amended Testing Progression

                  Data
                  • Dog 1 Donor 1 DoC 6/09/12
                    • BCM
                  • Dog 3 Donor 3  DoC 8/18/12
                    • BCM

                    Observations


                    Conclusion

                    seizure saliva on cotton

                    Question B IV



                    Question
                    Can odor produced during a   tonic clonic seizure  be captured from skin swab placed on  gauze pads be detected by dogs trained to identify specific odors

                    Research


                    • Research Index
                    • A IV


                    Hypothesis


                    Methods/Procedures


                    Equipment
                    • 24" scent wheel 
                    • 6 containers (clean tin cans)
                    • 6 glass shakers w.metal lids
                      • variable numbers of holes in lids
                    • Lazy Susan
                    Protocol
                    • run containers (cans) and shakers twice through the dishwasher prior to test
                    • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
                    • store in Ziplock plastic bags when not in use
                      • store "hot" shaker separately in its own Ziplock bag
                    Testing Instrument
                    • dog
                    • dog training
                      • Scent Discrimination
                      • Intro to Scent Wheel
                      • Qualifying Test Protocol
                      • Amended Testing Progression
                      • Trial Test

                    Data


                    • Dog 3 Donor 3  DoC 8/19/12
                      • BCM

                    Observations
                    Need 2 tests

                    Conclusion

                    Sample captured from skin swab and on gauze pads

                    Question C I



                    Question
                    Can a dog trained to identify chemical changes in the body distinguish between the odor of the saliva  in a person experiencing a  hypoglycemic event vs the odor of the saliva of the same person in a euglycemic state

                      Research


                      • Hypothesis A I
                      • Hypothesis B I


                      Hypothesis


                      Methods/Procedures


                      Equipment
                      • 24" scent wheel 
                      • 6 containers (clean tin cans)
                      • 6 glass shakers w.metal lids
                        • variable numbers of holes in lids
                      • Lazy Susan
                      Protocol
                      • run containers (cans) and shakers twice through the dishwasher prior to test
                      • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
                      • store in Ziplock plastic bags when not in use
                        • store "hot" shaker separately in its own Ziplock bag
                      Testing Instrument
                      • dog
                      • dog training
                        • Scent Discrimination
                        • Intro to Scent Wheel
                        • Qualifying Test Protocol
                        • Amended Testing Progression
                        • Trial Test

                      Data
                      • Dog 5 Donor 7  DoC 11/20/13
                        • BCM
                      • Dog 6 Dog 7  DoC   11/20/13
                        • BCM











                          Observations


                          Conclusion

                          all preceding criteria w/non event on wheel

                          Question C II



                          Question 
                          Can a dog trained to identify chemical changes in the body distinguish between the odor of the skin swab  in a person experiencing a  hypoglycemic event vs the odor of the skin swab of the same person in a euglycemic state


                          Research


                          Hypothesis


                          • ICAN Study
                          • A II
                          • B II

                          Methods/Procedures


                          Equipment
                          • 24" scent wheel 
                          • 6 containers (clean tin cans)
                          • 6 glass shakers w.metal lids
                            • variable numbers of holes in lids
                          • Lazy Susan
                          Protocol
                          • run containers (cans) and shakers twice through the dishwasher prior to test
                          • OR hand wash twice in hot soapy water and dishwashing detergent; rinse in hot water
                          • store in Ziplock plastic bags when not in use
                            • store "hot" shaker separately in its own Ziplock bag
                          Testing Instrument
                          • dog
                          • dog training
                            • Scent Discrimination
                            • Intro to Scent Wheel
                            • Qualifying Test Protocol
                            • Amended Testing Progression
                            • Trial Test

                          Data
                          • Dog 5 Donor 7  DoC  11/20/13
                            • BCM
                            • Dog 5 Donor 7  DoC  11/20/13
                              • 100%

                              Observations


                              Conclusion

                              all preceding criteria w/non-event on wheel